Anti-Human CD24 Recombinant Antibody (SWA11) (HB630107)

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货号	HB630107
品牌	abinScience
种属反应性	Human
应用	FCM, IHC, IF, RIA
同种型	IgG1, kappa
克隆号	SWA11
经测试应用	WB, FCM
靶标	Signal transducer CD24, CD24, CD24A, Small cell lung carcinoma cluster 4 antigen
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P25063
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Signal transducer CD24 is a ~8 kDa protein. May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. 1. Suzuki, T. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 166, 5567-77. PMID: 11313396
Note	For research use only.

SEC-HPLC

SEC-HPLC detection for Anti-Human CD24 Antibody (SWA11).
Bioactivity

Detects CD24 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Human CD24 Antibody (SWA11)
Flow Cytometry

Flow-cytometry using anti-human CD24 antibody. Human SK-BR-3 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD24 antibody monoclonal antibody (Catalog # HB630107 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD24 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD24 (Catalog No: HB630011)
Lane 2: negative control

产品说明书

Anti-Human CD24 Recombinant Antibody (SWA11).pdf

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Anti-Human CD24 Recombinant Antibody (SWA11) (HB630107)

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重组蛋白

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实验相关试剂

Anti-Human CD24 Recombinant Antibody (SWA11) (HB630107)

概述

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参考文献

产品说明书

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