| 货号 | HB630016 |
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| 品牌 | abinScience |
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| 种属反应性 | Human |
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| 应用 | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
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| 同种型 | IgG |
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| 克隆号 | ATG 031 |
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| 生物活性 | ATG-031 potently binds to CD24-postive tumor cells, while showing no binding with parental HEK293 cells or hRBC [1]. ATG-031 blocks the interaction between CD24 and Siglec-10 and induces potent macrophage-dependent tumor cell phagocytosis [1]. ATG-031 induces a potent antibody-dependent cellular cytotoxicity against HT-29 tumor cells with an EC 50 of 0.27 nM [3]. ATG-031 (1-3 mg/kg, i.v., biweekly) significantly inhibits tumor growth and induces tumor regression in MC38-hCD24 mouse model [1]. ATG-031 exhibits potent antitumor activity in vivo as a single agent in syngeneic models such as MC38, CT26, and 4T1 [3]. ATG-031 demonstrates in vivo synergism with chemotherapies and checkpoint inhibitors [3]. ATG-031 (3 mg/kg, i.v., 2 doses) significantly increases T cell-associated immune response at the cellular and single-cell transcriptome levels in C57BL/6 mice bearing MC38-hCD24 tumors [3]. |
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| 表达系统 | Mammalian cells |
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| 靶标 | Signal transducer CD24, CD24, CD24A, Small cell lung carcinoma cluster 4 antigen |
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| 内毒素水平 | < 10 EU/mg |
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| 纯度 | >95% purity as determined by SDS-PAGE. |
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| 纯化方式 | Protein A/G purified from cell culture supernatant. |
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| Accession号 | P25063 |
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| 状态 | Liquid |
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| 保存溶液 |
0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
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| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
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| 别名 | ATG-031, ATG031, ATG031 |
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| 背景 | Signal transducer CD24 is a ~8 kDa protein. May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. 1. Suzuki, T. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 166, 5567-77. PMID: 11313396 |
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| 文献 | 1. Peng Chen, et al., (2022). ATG-031, a first-in-class anti-CD24 antibody, showed potent preclinical anti-tumor efficacy by blocking “don’t-eat-me” signal. Journal for ImmunoTherapy of Cancer 2022. [HB630016]
2. Peng Chen, et al., Abstract 6641: ATG-031, a first-in-class humanized anti-CD24 antibody, demonstrates potent in vivo efficacy and repolarizes tumor-associated macrophages in the TME. Cancer Res 1 April 2023; 83 (7_Supplement): 6641. [HB630016]
3. Chen P, et al., 1337 The preclinical characterization and translational research of ATG-031, a first-in-class humanized anti-CD24 antibody, for the treatment of solid tumors and hematological malignanciesJournal for ImmunoTherapy of Cancer 2023;11 [HB630016] |
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| Note | For research use only. Not suitable for clinical or therapeutic use. |
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| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
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