Anti-Human CD277/BTN3A1 Recombinant Antibody (103.2#) (HT139107)

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货号	HT139107
品牌	abinScience
种属反应性	Human
应用	FCM, blocking
宿主	Mouse
同种型	IgG1, kappa
克隆号	103.2#
克隆类型	Monoclonal
靶标	BTF5, BTN3A1, Butyrophilin subfamily 3 member A1, CD277
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	O00481
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
别名	hu103.2
背景	Butyrophilin subfamily 3 member A1 (CD277/BTN3A1) is a ~57 kDa protein. Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.
Note	For research use only.

Bioactivity

Detects CD277/BTN3A1 in indirect ELISAs.
Flow-cytometry

Flow-cytometry using anti-human CD277 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD277 antibody monoclonal antibody (Catalog # HT139107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CD277/BTN3A1 Antibody (103.2#)
Western blot

Western blot analysis was performed using anti-CD277/BTN3A1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD277/BTN3A1 (Catalog No: HT139011)
Lane 2: negative control

产品说明书

Anti-Human CD277/BTN3A1 Recombinant Antibody (103.2#).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

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C₁ × V₁ = C₂ × V₂

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C₁ (起始浓度)

V₁ (起始体积)

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Anti-Human CD277/BTN3A1 Recombinant Antibody (103.2#) (HT139107)

概述

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参考文献

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抗体

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实验相关试剂

Anti-Human CD277/BTN3A1 Recombinant Antibody (103.2#) (HT139107)

概述

图片

参考文献

产品说明书

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