Anti-Human CD28 Recombinant Antibody (15E8#) (HY422207)

价格:

规格：

50ug
100ug

数量：

一键复制产品信息

概述
图片
参考文献
产品说明书

货号	HY422207
品牌	abinScience
种属反应性	Human
应用	FCM
同种型	IgG1
克隆号	15E8#
经测试应用	FCM
靶标	CD28, TP44, T-cell-specific surface glycoprotein CD28
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P10747
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	T-cell-specific surface glycoprotein CD28 is a ~25 kDa protein. Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells. 1. Gimmi, CD. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 6575-9. PMID: 1650475 2. Raab, M. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 8891-5. PMID: 7568038 3. Skånland, SS. et al. (2014) The Biochemical journal 460, 399-410. PMID: 24665965 4. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291 5. Wu, H. et al. (2022) Structure (London, England : 1993) 30, 803-812.e5. PMID: 35397202 6. Kong, KF. et al. (2011) Nature immunology 12, 1105-12. PMID: 21964608 7. Blotta, MH. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 3133-40. PMID: 8617933
Note	For research use only.

Bioactivity

SEC-HPLC detection for Anti-Human CD28 Antibody (15E8#).
Flow CytoMetry

Flow-cytometry using anti-human CD28 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD28 monoclonal antibody (Catalog: HY422207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human CD28 Recombinant Antibody (15E8#).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CD28 Recombinant Antibody (15E8#) (HY422207)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CD28 Recombinant Antibody (15E8#) (HY422207)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价