Anti-Human CD28 Reference Antibody (Pegrizeprument, RUO) (HY422056)

价格:

规格：

100ug
1mg
5mg
10mg
25mg
50mg
100mg

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HY422056

品牌

abinScience

描述

Pegrizeprument (HY422056) is a research-grade recombinant antibody targeting CD28. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

种属反应性

Human

应用

ELISA, Bioactivity: FACS, Functional assay, Research in vivo, WB

宿主

Chimeric

同种型

Immunoglobulin Fab VH-G1CH1h_L-kappa

表达系统

Mammalian cells

克隆类型

Monoclonal

经测试应用

靶标

CD28, TP44, T-cell-specific surface glycoprotein CD28

内毒素水平

< 10 EU/mg

纯度

>95% purity as determined by SDS-PAGE.

纯化方式

Protein A/G purified from cell culture supernatant.

Accession号

P10747

状态

Liquid

保存溶液

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

别名

2829316-24-5

背景

T-cell-specific surface glycoprotein CD28 is a ~25 kDa protein. Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells.

1. Gimmi, CD. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 6575-9. PMID: 1650475

2. Raab, M. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 8891-5. PMID: 7568038

3. Skånland, SS. et al. (2014) The Biochemical journal 460, 399-410. PMID: 24665965

4. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291

5. Wu, H. et al. (2022) Structure (London, England : 1993) 30, 803-812.e5. PMID: 35397202

6. Kong, KF. et al. (2011) Nature immunology 12, 1105-12. PMID: 21964608

7. Blotta, MH. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 3133-40. PMID: 8617933

Note

For research use only. Not suitable for clinical or therapeutic use.

图片

SDS-PAGE

SDS-PAGE for Research Grade Pegrizeprument.
Western blot

Western blot analysis was performed using anti-CD28 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD28 (Catalog No: HY422011)
Lane 2: Negative control

参考文献

产品说明书

Anti-Human CD28 Reference Antibody (Pegrizeprument, RUO).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CD28 Reference Antibody (Pegrizeprument, RUO) (HY422056)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CD28 Reference Antibody (Pegrizeprument, RUO) (HY422056)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价