Anti-Human CD34 Recombinant Antibody (My10) (HB766107)

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货号	HB766107
品牌	abinScience
种属反应性	Human
应用	FCM, WB
同种型	IgG1, kappa
克隆号	My10
经测试应用	FCM
靶标	CD34, Hematopoietic progenitor cell antigen CD34
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P28906
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Hematopoietic progenitor cell antigen CD34 is a ~40 kDa protein. Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Note	For research use only.

SEC-HPLC

SEC-HPLC detection for Anti-Human CD34 Antibody (My10).
Bioactivity

Detects CD34 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD34 antibody. Human MOLT-4 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD34 antibody monoclonal antibody (Catalog # HB766107 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using anti-human CD34 antibody. KG-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD34 monoclonal antibody (Catalog HB766107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CD34 Recombinant Antibody (My10)

产品说明书

Anti-Human CD34 Recombinant Antibody (My10).pdf

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Anti-Human CD34 Recombinant Antibody (My10) (HB766107)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CD34 Recombinant Antibody (My10) (HB766107)

概述

图片

参考文献

产品说明书

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