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Anti-Human CD34 Recombinant Antibody (QBEND-10) (HB766207)

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概述
货号HB766207
品牌abinScience
种属反应性Human
应用FCM
同种型IgG1, lambda
克隆号QBEND-10
经测试应用FCM
靶标CD34, Hematopoietic progenitor cell antigen CD34
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P28906
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Hematopoietic progenitor cell antigen CD34 is a ~40 kDa protein. Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

NoteFor research use only.
图片
  • Bioactivity

    SEC-HPLC detection for Anti-Human CD34 Antibody (QBEND-10).

  • Flow Cytometry

    Flow-cytometry using anti-human CD34 antibody.CD34 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD34 antibody monoclonal antibody (Catalog # HB766207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human CD34 antibody.KG-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD34 antibody monoclonal antibody (Catalog # HB766207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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