Anti-Human CD8 alpha Recombinant Antibody (mab1) (HF815107)

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100ug

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货号	HF815107
品牌	abinScience
种属反应性	Human
应用	FCM
同种型	IgG1, kappa
克隆号	mab1
经测试应用	FCM
靶标	T-cell surface glycoprotein CD8 alpha chain, CD8a, T-lymphocyte differentiation antigen T8/Leu-2, MAL, CD8A
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P01732
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	T-cell surface glycoprotein CD8 alpha chain (CD8a/Lyt2) is a ~25 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.
Note	For research use only.

Flow-cytometry

Flow-cytometry using anti- human CD8 antibody.
Human RPMI-8226 cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD8 antibody monoclonal antibody (Catalog # HF815107 , Red Histogram) at a concentration of 20ug/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

SEC-HPLC detection for Anti-Human CD8A Antibody (mab1).

产品说明书

Anti-Human CD8 alpha Recombinant Antibody (mab1).pdf

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Anti-Human CD8 alpha Recombinant Antibody (mab1) (HF815107)

概述

图片

参考文献

产品说明书

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重组蛋白

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实验相关试剂

Anti-Human CD8 alpha Recombinant Antibody (mab1) (HF815107)

概述

图片

参考文献

产品说明书

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