Anti-Mouse CTLA-4 Recombinant Antibody (H11) (MB651107)

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概述

货号	MB651107
品牌	abinScience
描述	Anti-Mouse CTLA-4 Recombinant Antibody (H11) (MB651107) is a mouse monoclonal antibody detecting CTLA-4 in ELISA, FCM. Suitable for Mouse. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Multi-Application — Validated across multiple applications.
种属反应性	Mouse
应用	ELISA, FCM
宿主	Mouse
克隆类型	Monoclonal
同种型	VHH-mFc (IgG2a)
克隆号	H11
经测试应用	FCM
靶标	CD152, CTLA-4, CTLA4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4
内毒素水平	< 10 EU/mg as determined by LAL method.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P09793
状态	Liquid
保存溶液	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景	Cytotoxic T-lymphocyte protein 4 (CD152/CTLA4) is a ~24 kDa protein. Inhibitory receptor acting as a major negative regulator of T-cell responses. Acts as a decoy receptor: the affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. CD152 is the therapeutic target of ipilimumab (Yervoy).
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-mouse CD152 antibody.CD152 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD152 antibody monoclonal antibody (Catalog # MB651107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

Detects recombinant mouse CD152/CTLA4 (Catalog No: MB651011) in indirect ELISA

参考文献

产品说明书

Anti-Mouse CTLA-4 Recombinant Antibody (H11).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

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C₂ (终浓度)

V₂ (终体积)

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Anti-Mouse CTLA-4 Recombinant Antibody (H11) (MB651107)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse CTLA-4 Recombinant Antibody (H11) (MB651107)

概述

图片

参考文献

产品说明书

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