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Anti-Mouse SHH Recombinant Antibody (6D7) (MS826107)

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概述
货号MS826107
品牌abinScience
种属反应性Human, Mouse
应用ELISA, FCM
同种型IgG1, kappa
克隆号6D7
靶标ShhNC, Sonic hedgehog protein, Shh N-terminal processed signaling domains, SHH, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhN, ShhNp, HHG-1
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号Q62226
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Sonic hedgehog protein (SHH) is a ~47 kDa protein. The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the reticulum endoplasmic. Once cleaved, ShhC is degraded in the endoplasmic reticulum.

1. Roelink, H. et al. (1995) Cell 81, 445-55. PMID: 7736596
2. Bumcrot, DA. et al. (1995) Molecular and cellular biology 15, 2294-303. PMID: 7891723
3. Porter, JA. et al. (1996) Science (New York, N.Y.) 274, 255-9. PMID: 8824192
4. Chen, X. et al. (2011) The Journal of cell biology 192, 825-38. PMID: 21357747
NoteFor research use only.
图片
  • SDS-PAGE

    SDS-PAGE for Anti-Mouse SHH Antibody (6D7)

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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