Anti-Human CEACAM-1/CD66a Recombinant Antibody (SAA0761) (HB880107)

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50ug
100ug

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货号	HB880107
品牌	abinScience
种属反应性	Human
应用	ELISA, FCM, WB
同种型	IgG2a
克隆号	SAA0761
靶标	CD66a, BGP-1, Biliary glycoprotein 1, CEACAM1, Carcinoembryonic antigen-related cell adhesion molecule 1, BGP, BGP1
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P13688
状态	Liquid
保存溶液	0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Cell adhesion molecule CEACAM1 (CD66a) is a ~57 kDa protein. Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner. Plays a role as coinhibitory receptor in immune response, insulin action and also functions as an activator during angiogenesis. Its coinhibitory receptor function is phosphorylation- and PTPN6 -dependent, which in turn, suppress signal transduction of associated receptors by dephosphorylation of their downstream effectors. Plays a role in immune response, of T cells, natural killer (NK) and neutrophils. Upon TCR/CD3 complex stimulation, inhibits TCR-mediated cytotoxicity by blocking granule exocytosis by mediating homophilic binding to adjacent cells, allowing interaction with and phosphorylation by LCK and interaction with the TCR/CD3 complex which recruits PTPN6 resulting in dephosphorylation of CD247 and ZAP70. 1. Chen, Z. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 6085-93. PMID: 18424730 2. Hosomi, S. et al. (2013) European journal of immunology 43, 2473-83. PMID: 23696226 3. Huang, YH. et al. (2015) Nature 517, 386-90. PMID: 25363763 4. Klaile, E. et al. (2005) Journal of cell science 118, 5513-24. PMID: 16291724
Note	For research use only.

Flow Cytometry

Flow-cytometry using anti-human CD66a antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD66a antibody monoclonal antibody (Catalog # HB880107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD66a antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD66a antibody monoclonal antibody (Catalog # HB880107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD66a monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD66a (Catalog No: HB880011)
Lane 2: negative control

产品说明书

Anti-Human CEACAM-1/CD66a Recombinant Antibody (SAA0761).pdf

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Anti-Human CEACAM-1/CD66a Recombinant Antibody (SAA0761) (HB880107)

概述

图片

参考文献

产品说明书

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重组蛋白

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实验相关试剂

Anti-Human CEACAM-1/CD66a Recombinant Antibody (SAA0761) (HB880107)

概述

图片

参考文献

产品说明书

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