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Anti-Human CD30/TNFRSF8 Recombinant Antibody (SAA1390) (HB038407)

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概述
货号HB038407
品牌abinScience
种属反应性Human
应用FCM
同种型IgG1, kappa
克隆号SAA1390
经测试应用FCM
靶标CD30L receptor, Ki-1 antigen, CD30, TNFRSF8, D1S166E, Tumor necrosis factor receptor superfamily member 8, Lymphocyte activation antigen CD30
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P28908
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 0.09% Sodium azide.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Tumor necrosis factor receptor superfamily member 8 (CD30/TNFRSF8) is a ~63 kDa protein. Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. CD30 is the therapeutic target of brentuximab vedotin (Adcetris).

1. Smith, CA. et al. (1993) Cell 73, 1349-60. PMID: 8391931
2. Aizawa, S. et al. (1997) The Journal of biological chemistry 272, 2042-5. PMID: 8999898
NoteFor research use only.
图片
  • Flow Cytometry

    Flow-cytometry using anti-human CD30 antibody.CD30 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD30 antibody monoclonal antibody (Catalog # HB038407 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human CD30 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD30 antibody monoclonal antibody (Catalog # HB038407 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using anti-human CD30 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD30 monoclonal antibody (Catalog: HB038407) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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