Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (5F24) (HB796207)

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货号	HB796207
品牌	abinScience
种属反应性	Human
应用	FCM, WB
同种型	IgG2a, kappa
克隆号	5F24
经测试应用	WB, FCM
靶标	CD27L receptor, TNFRSF7, T14, CD27 antigen, Tumor necrosis factor receptor superfamily member 7, CD27, T-cell activation antigen CD27
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P26842
状态	Liquid
保存溶液	0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	CD27 antigen is a ~29 kDa protein. Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue. 1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863 2. Jaeger-Ruckstuhl, CA. et al. (2024) Immunity 57, 287-302.e12. PMID: 38354704
Note	For research use only.

Bioactivity

Detects Human CD27 in indirect ELISAs.
Flow CytoMetry

Flow-cytometry using anti-human CD27 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD27 monoclonal antibody (Catalog: HB796207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD27 monoclonal antibody at 1µg/ml on various samples.
Lane 1: recombinant human CD27 (Catalog No: HB796011)
Lane 2: negative control

产品说明书

Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (5F24).pdf

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Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (5F24) (HB796207)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

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实验相关试剂

Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (5F24) (HB796207)

概述

图片

参考文献

产品说明书

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