Anti-Human CD275/ICOSLG Recombinant Antibody (SAA0089), FITC (HF564117)

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货号	HF564117
品牌	abinScience
种属反应性	Human
应用	FCM
宿主	Human
同种型	IgG2, kappa
克隆号	SAA0089
偶联物	FITC
克隆类型	Monoclonal
靶标	B7RP1, B7-related protein 1, CD275, ICOSL, ICOSLG, KIAA0653, B7 homolog 2, ICOS ligand, B7RP-1, B7H2, B7-H2, B7-like protein Gl50
内毒素水平	Please contact with the lab for this information.
相关产品	Human IgG2 Recombinant Isotype Control Antibody (HyHEL-10), FITC
Accession号	O75144
状态	Liquid
保存溶液	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	ICOS ligand (CD275/ICOSLG) is a ~33 kDa protein. Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion. Also induces B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. In endothelial cells, required for proper neutrophil transmigration in response to chemoattractants, such as CXCL8/IL8 or N-formyl-methionyl peptides (fMLP). 1. Yoshinaga, SK. et al. (2000) International immunology 12, 1439-47. PMID: 11007762 2. Wang, S. et al. (2000) Blood 96, 2808-13. PMID: 11023515 3. Roussel, L. et al. (2018) The Journal of experimental medicine 215, 3151-3164. PMID: 30498080
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Flow CytoMetry

Flow-cytometry using FITC anti-human CD275 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD275 monoclonal antibody (Catalog: HF564117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human CD275/ICOSLG Recombinant Antibody (SAA0089), FITC.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

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C₁ × V₁ = C₂ × V₂

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C₁ (起始浓度)

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Anti-Human CD275/ICOSLG Recombinant Antibody (SAA0089), FITC (HF564117)

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参考文献

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重组蛋白

抗体

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实验相关试剂

Anti-Human CD275/ICOSLG Recombinant Antibody (SAA0089), FITC (HF564117)

概述

图片

参考文献

产品说明书

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