Anti-Human B7-H2 Reference Antibody (Prezalumab, RUO) (HF564016)

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概述

货号	HF564016
品牌	abinScience
描述	Prezalumab (HF564016) is a research-grade recombinant antibody targeting CD275. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
种属反应性	Human
应用	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
同种型	IgG2-kappa
表达系统	Mammalian cells
靶标	B7RP1, B7-related protein 1, CD275, ICOSL, ICOSLG, KIAA0653, B7 homolog 2, ICOS ligand, B7RP-1, B7H2, B7-H2, B7-like protein Gl50
内毒素水平	< 10 EU/mg
纯度	>95% purity as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	O75144
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	AMG557, MEDI-5872, 1523164-68-2
背景	ICOS ligand (CD275/ICOSLG) is a ~33 kDa protein. Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion. Also induces B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. In endothelial cells, required for proper neutrophil transmigration in response to chemoattractants, such as CXCL8/IL8 or N-formyl-methionyl peptides (fMLP). 1. Yoshinaga, SK. et al. (2000) International immunology 12, 1439-47. PMID: 11007762 2. Wang, S. et al. (2000) Blood 96, 2808-13. PMID: 11023515 3. Roussel, L. et al. (2018) The Journal of experimental medicine 215, 3151-3164. PMID: 30498080
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG2 Recombinant Isotype Control Antibody (HyHEL-10) (HV988107)

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SEC-HPLC

SEC-HPLC detection for Research Grade Prezalumab.
Bioactivity

Detects CD275/ICOSLG in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Prezalumab.
Flow CytoMetry

Flow-cytometry using anti-human CD275 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD275 monoclonal antibody (Catalog: HF564016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献

产品说明书

Anti-Human B7-H2 Reference Antibody (Prezalumab, RUO).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

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Anti-Human B7-H2 Reference Antibody (Prezalumab, RUO) (HF564016)

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参考文献

产品说明书

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Anti-Human B7-H2 Reference Antibody (Prezalumab, RUO) (HF564016)

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参考文献

产品说明书

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