Anti-Mouse CD25/IL2R alpha Recombinant Antibody (PC61/PC-61.5.3), FITC (MF996117)

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货号	MF996117
品牌	abinScience
种属反应性	Mouse
应用	FCM
宿主	Rat
同种型	IgG1, lambda
克隆号	PC61/PC-61.5.3
偶联物	FITC
克隆类型	Monoclonal
经测试应用	FCM
靶标	Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55, CD25, Il2ra, Il2r
内毒素水平	Please contact with the lab for this information.
纯度	>90% as determined by SDS-PAGE.
相关产品	Rat IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P01590
状态	Liquid
保存溶液	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
稳定性和存储	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	Interleukin-2 receptor subunit alpha (CD25/IL2RA) is a ~30 kDa protein. Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Flow CytoMetry

Flow-cytometry using FITC anti-Mouse CD25 antibody. Unstimulated cells (blue Histogram) and 3-day CoA-stimulated Mouse spleen cells (Yellow Histogram) were stained with an FITC anti-Mouse CD25 monoclonal antibody (Catalog: MF996117) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Mouse CD25/IL2R alpha Recombinant Antibody (PC61/PC-61.5.3), FITC.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

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C₁ × V₁ = C₂ × V₂

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C₁ (起始浓度)

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C₂ (终浓度)

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Anti-Mouse CD25/IL2R alpha Recombinant Antibody (PC61/PC-61.5.3), FITC (MF996117)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse CD25/IL2R alpha Recombinant Antibody (PC61/PC-61.5.3), FITC (MF996117)

概述

图片

参考文献

产品说明书

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