Anti-Human CD28 Recombinant Antibody (SAA0010), APC (HY422137)

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货号	HY422137
品牌	abinScience
种属反应性	Human
应用	ELISA, FCM, WB
宿主	Human
同种型	IgG4
克隆号	SAA0010
偶联物	APC
克隆类型	Monoclonal
经测试应用	FCM
靶标	CD28, TP44, T-cell-specific surface glycoprotein CD28, RHC83401
内毒素水平	Please contact with the lab for this information.
相关产品	Human IgG4 (S228P) Recombinant Isotype Control Antibody (13R4), APC
Accession号	P10747
状态	Liquid
保存溶液	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	T-cell-specific surface glycoprotein CD28 is a ~25 kDa protein. Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells. 1. Gimmi, CD. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 6575-9. PMID: 1650475 2. Raab, M. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 8891-5. PMID: 7568038 3. Skånland, SS. et al. (2014) The Biochemical journal 460, 399-410. PMID: 24665965 4. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291 5. Wu, H. et al. (2022) Structure (London, England : 1993) 30, 803-812.e5. PMID: 35397202 6. Kong, KF. et al. (2011) Nature immunology 12, 1105-12. PMID: 21964608 7. Blotta, MH. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 3133-40. PMID: 8617933
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Flow Cytometry

Flow-cytometry using APC anti-human CD28 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Green Histogram) or an APC anti-human CD28 antibody monoclonal antibody (Catalog # HY422137, Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human CD28 Recombinant Antibody (SAA0010), APC.pdf

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Anti-Human CD28 Recombinant Antibody (SAA0010), APC (HY422137)

概述

图片

参考文献

产品说明书

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重组蛋白

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实验相关试剂

Anti-Human CD28 Recombinant Antibody (SAA0010), APC (HY422137)

概述

图片

参考文献

产品说明书

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