

| 货号 | ICH3042CF | ||||
|---|---|---|---|---|---|
| 品牌 | IchorBio | ||||
| 描述 |
Untouched Human Classical Monocyte Isolation Kit (ICH3042CF) is a antibody targeting Classical Monocyte (CD14+). Reactive with Human. Validated for Cell isolation / separation (flow cytometry, cell culture, functional & cell-therapy research).
Highlights
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| 种属反应性 | Human | ||||
| 应用 | Cell isolation / separation (flow cytometry, cell culture, functional & cell-therapy research) | ||||
| 克隆类型 | Monoclonal | ||||
| 经测试应用 | Cell isolation / separation (flow cytometry, cell culture, functional & cell-therapy research) | ||||
| 靶标 | Classical Monocyte (CD14+) | ||||
| 特异性 | Classical Monocyte (CD14+) cells | ||||
| Accession号 | P08571 | ||||
| 状态 | Liquid | ||||
| 运输 | Blue Ice | ||||
| 产品使用信息 |
| ||||
| 稳定性和存储 | Store at 2–8°C; do not freeze | ||||
| 规格 | Size: 100T | ||||
| 背景 | Classical monocytes (CD14+CD16-) are the predominant monocyte subset in peripheral blood, serving as precursors for macrophages and dendritic cells and playing key roles in innate immunity, phagocytosis, and inflammatory responses. Their differentiation potential and functional state are highly sensitive to the isolation method used, making unmanipulated recovery essential for downstream applications such as macrophage differentiation. The Ichorbio Untouched Human Classical Monocyte Isolation Kit purifies human CD14+CD16- classical monocytes from PBMCs via column-free negative (untouched) selection, using a biotin-conjugated antibody cocktail and magnetic beads to label and sequester non-target cells, allowing untouched classical monocytes to be recovered directly from the supernatant. This yields high-purity, untouched cells free of surface-bound antibodies or beads, with high viability and native phenotype preserved for sensitive downstream applications. CD14 is expressed on classical (CD14++CD16-) monocytes and macrophages, and at lower levels on granulocytes and dendritic cells; a canonical marker of the monocyte/macrophage lineage. | ||||
| Note | Research Use Only (RUO) |

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