

| 货号 | HB714137 |
|---|---|
| 品牌 | abinScience |
| 种属反应性 | Human |
| 应用 | FCM, WB |
| 宿主 | Mouse |
| 同种型 | IgG1, kappa |
| 克隆号 | SAA0045 |
| 偶联物 | APC |
| 克隆类型 | Monoclonal |
| 经测试应用 | FCM |
| 靶标 | IL-3R subunit alpha, IL-3 receptor subunit alpha, CD123, Interleukin-3 receptor subunit alpha, IL3R, IL-3R-alpha, IL3RA, IL-3RA |
| 内毒素水平 | Please contact with the lab for this information. |
| 相关产品 | Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), APC |
| Accession号 | P26951 |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| 稳定性和存储 | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| 背景 | Interleukin-3 receptor subunit alpha (CD123/IL-3Rα) is a ~43 kDa protein. Cell surface receptor for IL3 expressed on hematopoietic progenitor cells, monocytes and B-lymphocytes that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Ligand stimulation rapidly induces hetrodimerization with IL3RB, phosphorylation and enzyme activity of effector proteins such as JAK2 and PI3K that play a role in signaling cell proliferation and differentiation. Activation of JAK2 leads to STAT5-mediated transcriptional program. 1. Huang, S. et al. (1999) Stem cells (Dayton, Ohio) 17, 265-72. PMID: 10527461 |
| Note | For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only. |

Flow-cytometry using APC anti-Human CD123 antibody. KG-1 cells were stained with an irrelevant antibody (Green Histogram) or an APC anti-Human CD123 antibody monoclonal antibody (Catalog # HB714137 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.



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