CD4+ Helper T-Cell Depletion & Validation Kit (ICH8264-50PE)

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

The CD4+ Helper T-Cell Depletion and Validation Kit (ICH8264) is a paired-reagent set designed for controlled depletion of CD4+ helper T cells in mouse models and independent confirmation of that depletion by flow cytometry. Both listed components are Rat IgG2b, Kappa antibodies reactive against mouse CD4, consistent with a kit format pairing a depleting anti-CD4 clone with a non-competing detection/validation clone so that residual CD4+ cells can be tracked without epitope masking by the depleting antibody. Reagents are supplied research grade (RUO), typically low endotoxin and formulated for in-vivo administration and bulk use. This kit-style product is intended to standardise depletion experiments: the depleting antibody removes the CD4 compartment while the validation antibody, binding a distinct epitope, enables accurate quantification of depletion efficiency in blood, spleen, or lymph node. It is not a therapeutic drug and is not intended for clinical use. Because exact clone identities and endotoxin specifications are not stated on the card, confirm component clones, formats, and concentrations with ichor.bio before designing a study. CD4 is a single-chain transmembrane glycoprotein of the immunoglobulin superfamily expressed on helper T cells, and at lower levels on some monocytes, macrophages, and dendritic cells. It acts as a co-receptor for the T-cell receptor, binding the non-polymorphic beta-2 region of MHC class II molecules on antigen-presenting cells and recruiting the tyrosine kinase Lck to stabilise the immunological synapse and amplify TCR signalling. CD4+ helper T cells orchestrate adaptive immunity by providing help to B cells, licensing dendritic cells, and secreting polarising cytokines (Th1, Th2, Th17, Tfh, and regulatory subsets). Because of this central coordinating role, antibody-mediated depletion of CD4+ cells is a widely used approach to dissect helper T-cell contributions to infection, autoimmunity, transplantation, and antitumour immunity in mouse models.