Macrophage Depletion & Validation Kit (ICH8266-25PE)

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

The Macrophage Depletion and Validation Kit (ICH8266) is a research-use-only toolset designed to deplete macrophages in mouse models and to confirm the extent and specificity of that depletion. The kit is built around depleting antibodies of rat origin, indicated by the listed isotypes (Rat IgG2a Kappa and Rat IgG2b Kappa), typically paired with detection or validation reagents so investigators can both remove the target population and verify the outcome by flow cytometry or histology. It is intended for use in mouse, is supplied at research grade, and is not a clinical product. Macrophage-depletion approaches are widely used to interrogate the contribution of these cells to immunity, inflammation, tumor biology, tissue repair, and clearance of particulates or pathogens. Because incomplete or off-target depletion is a common source of experimental artifact, a paired validation component is valuable: it lets researchers document depletion efficiency, monitor repopulation kinetics, and include appropriate controls. As with all in-vivo depletion strategies, the choice of dose, route, and schedule depends heavily on the tissue compartment targeted and the specific model. Exact kit contents and reagent formats should be confirmed against the current ichor.bio product documentation. Macrophages are versatile myeloid cells found across essentially every tissue, existing both as long-lived tissue-resident populations (for example Kupffer cells, alveolar macrophages, microglia) and as monocyte-derived cells recruited during inflammation. They perform phagocytosis, antigen presentation, cytokine and chemokine production, and tissue remodeling, and they polarize along a functional spectrum often summarized as pro-inflammatory versus reparative or immunosuppressive. In disease, tumor-associated macrophages can promote angiogenesis, immune evasion, and metastasis, making their depletion a common experimental strategy. Depleting antibodies in kits like this typically target surface markers enriched on macrophages or their monocyte precursors; the two rat isotypes suggested here are consistent with a combination approach. Because macrophage subsets differ markedly in ontogeny, marker expression, turnover, and susceptibility to depletion, the achievable degree of depletion is population- and tissue-dependent, which is precisely why paired validation reagents are included.