Natural Killer (NK) Cell Depletion & Validation Kit (ICH8268-25F)

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

The Natural Killer (NK) Cell Depletion and Validation Kit (ICH8268) is a research-grade, low-endotoxin toolkit assembled for the functional depletion of NK cells in mouse models and for the parallel confirmation of that depletion by flow cytometry or tissue staining. It pairs an in-vivo depleting antibody with the reagents needed to verify the extent and duration of NK cell removal, so investigators can both perturb and quantify the NK compartment within a single, consistency-controlled lot. The listed isotypes (Mouse IgG2a Kappa and Rat IgM Kappa) reflect the distinct components: an IgG2a-format depleting clone and an IgM-format reagent used in the validation workflow. All materials are supplied for research use only, are not therapeutic or clinical products, and are manufactured to in-vivo standards with low endotoxin. The kit is intended to support studies of NK-cell contributions to tumour immunosurveillance, antiviral defence, antibody-dependent cellular cytotoxicity, transplant rejection, and innate-adaptive crosstalk. Because kit composition and exact clones determine correct use, confirm the datasheet before designing depletion and readout experiments. Natural killer (NK) cells are innate lymphocytes that survey for stressed, infected, and transformed cells without prior antigen sensitisation. Their activity is governed by the balance of activating (e.g., NKG2D, NCRs, CD16/FcgammaRIII) and inhibitory (Ly49, NKG2A) receptor signals, allowing "missing-self" and "induced-self" recognition. Once triggered, NK cells kill via perforin and granzyme release and death-receptor ligands, and secrete IFN-gamma and other cytokines that shape downstream T-cell and myeloid responses. In mice, NK cells are commonly identified by NK1.1 (in permissive strains such as C57BL/6), CD49b (DX5), and NKp46, and depletion strategies typically target these lineage markers. Because NK cells also mediate antibody-dependent cellular cytotoxicity, they are central to studies of monoclonal-antibody efficacy, tumour control, viral clearance, and allograft rejection, making reproducible depletion and rigorous validation important experimental controls.