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Anti-Human CD83 Recombinant Antibody (60B10), PE (HX237127)

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规格:
  • 50T
  • 100T
数量:
  • 概述

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  • 参考文献

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概述
货号HX237127
品牌abinScience
种属反应性Human
应用FCM
宿主Mouse
同种型IgG1, kappa
克隆号60B10
偶联物PE
克隆类型Monoclonal
经测试应用FCM
靶标CD83, hCD83, CD83 antigen, B-cell activation protein, Cell surface protein HB15
内毒素水平Please contact with the lab for this information.
相关产品 Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), PE
Accession号Q01151
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Store at 4°C for 12 months. Protect from light. Do not freeze.
背景

CD83 antigen is a ~23 kDa protein. Transmembrane glycoprotein predominantly found on the surface of many immune cells including dendritic cells or lymphocytes that plays various roles in immune response regulation. Plays an essential role in CD4(+) T-selection, differentiation and stability by regulating the activity of the major E3 ubiquitin ligase responsible for controlling MHCII trafficking MARCHF8. Also inhibits MARCHF1 association with MHCII or CD86 to prevent their ubiquitination and subsequent degradation. In addition, acts as an important modulator of protective responses against acute infections.

1. Tze, LE. et al. (2011) The Journal of experimental medicine 208, 149-65. PMID: 21220452
NoteFor flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
图片
  • Flow CytoMetry

    Flow-cytometry using PE anti-Mouse CD83 antibody. Unstimulated cells (blue Histogram) and 3-day LPS-stimulated Mouse spleen cells (Yellow Histogram) were stained with an FITC anti-Mouse CD83 monoclonal antibody (Catalog: HX237127) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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