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Anti-Human DPEP3 Recombinant Antibody (B43), PE (HV426127)

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规格:
  • 50T
  • 100T
数量:
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概述
货号HV426127
品牌abinScience
种属反应性Human
应用ELISA, FCM
宿主Mouse
同种型IgG2a, kappa
克隆号B43
偶联物PE
克隆类型Monoclonal
靶标DPEP3, Dipeptidase 3
内毒素水平Please contact with the lab for this information.
相关产品 Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173), PE
纯化方式Affinity chromatography.
Accession号Q9H4B8
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Store at 4°C for 12 months. Protect from light. Do not freeze.
背景

Dipeptidase 3 (DPEP3) is a ~53 kDa protein. Lacks dipeptidase activity and is unable to hydrolyze cystinyl-bis-glycine, leukotriene D4 and the beta-lactam antibiotic imipenem. The absence of activity may be due to the inability of asparagine (instead of aspartate found in DPEP1/2) at position 359 to function as the acid/base catalyst and activate the nucleophilic water/hydroxide. A tyrosine (instead of histidine) at position 269 reduces affinity for the beta zinc and may cause substrate steric hindrance.

1. Hayashi, K. et al. (2020) Journal of structural biology 211, 107512. PMID: 32325220
NoteFor flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
图片
  • Flow Cytometry

    Flow-cytometry using PE anti-Human CD19 antibody. Raji cells were stained with an irrelevant antibody (Green Histogram) or a PE anti-Human CD19 antibody monoclonal antibody (Catalog # HV426127 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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