Anti-Human Podocalyxin Like Recombinant Antibody (Mab47) (HT033107)

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货号	HT033107
品牌	abinScience
种属反应性	Human
应用	ELISA, FCM, IHC, Neutralization
同种型	IgG2a, kappa
克隆号	Mab47
靶标	PCLP1, Podocalyxin-like protein 1, GCTM-2 antigen, Gp200, PODXL, Podocalyxin, PC, PCLP, PCLP-1
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	O00592
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Podocalyxin (PODXL) is a ~58 kDa protein. Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis.
Note	For research use only.

Bioactivity

Detects recombinant human PODXL (Cat No.: HT033012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-PODXL monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human PODXL (Catalog No: HT033012)
Lane 2: negative control
Flow cytometry

Flow-cytometry using anti-human PODXL antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human PODXL monoclonal antibody (Catalog HT033107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human Podocalyxin Like Recombinant Antibody (Mab47).pdf

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Anti-Human Podocalyxin Like Recombinant Antibody (Mab47) (HT033107)

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实验相关试剂

Anti-Human Podocalyxin Like Recombinant Antibody (Mab47) (HT033107)

概述

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参考文献

产品说明书

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