Anti-Human CD276/B7-H3 Reference Antibody (Mirzotamab, RUO) (HV612036)

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概述

货号	HV612036
品牌	abinScience
描述	Mirzotamab (HV612036) is a research-grade recombinant antibody targeting CD276. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
种属反应性	Human
应用	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
同种型	IgG1-kappa
表达系统	Mammalian cells
经测试应用	WB, FCM
靶标	CD276, B7-H3, Costimulatory molecule, B7H3, 4Ig-B7-H3, B7 homolog 3, CD276 antigen
内毒素水平	< 10 EU/mg
纯度	>95% purity as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	Q5ZPR3
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	ABBV-155, 2229859-11-2
背景	CD276 antigen (B7-H3) is a ~57 kDa protein. May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. CD276 is the therapeutic target of omburtamab (clinical).
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

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SEC-HPLC

SEC-HPLC detection for Research Grade Mirzotamab.
Bioactivity

Detects CD276/B7-H3 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Mirzotamab.
Flow CytoMetry

Flow-cytometry using APC anti-human CD276 antibody. PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD276 monoclonal antibody (Catalog: HV612036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human CD276 antibody. PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD276 monoclonal antibody (Catalog: HV612036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD276 antibody. PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD276 monoclonal antibody (Catalog: HV612036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD276 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD276 (Catalog No: HV612011)

参考文献

产品说明书

Anti-Human CD276/B7-H3 Reference Antibody (Mirzotamab, RUO).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

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Anti-Human CD276/B7-H3 Reference Antibody (Mirzotamab, RUO) (HV612036)

概述

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参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CD276/B7-H3 Reference Antibody (Mirzotamab, RUO) (HV612036)

概述

图片

参考文献

产品说明书

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