Anti-Human EpCAM/TROP1 Reference Antibody (Citatuzumab, RUO) (HB104016)

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概述
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产品说明书

概述

货号	HB104016
品牌	abinScience
描述	Citatuzumab (HB104016) is a research-grade recombinant antibody targeting CD326. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
种属反应性	Human
应用	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
同种型	IgG1-kappa
表达系统	Mammalian cells
经测试应用	FCM
靶标	Epithelial glycoprotein 314, hEGP314, Major gastrointestinal tumor-associated protein GA733-2, EGP314, TACSTD1, Adenocarcinoma-associated antigen, TROP1, EPCAM, Epithelial glycoprotein, KS 1/4 antigen, Tumor-associated calcium signal transducer 1, M4S1, CD326, Ep-CAM, EGP, Epithelial cell adhesion molecule, M1S2, KSA, Cell surface glycoprotein Trop-1, GA733-2, Epithelial cell surface antigen, MIC18
内毒素水平	< 10 EU/mg
纯度	>95% purity as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P16422
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	VB6-845, 945228-49-9
背景	Epithelial cell adhesion molecule (CD326/EPCAM) is a ~34 kDa protein. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 (LALAPG) Recombinant Isotype Control Antibody (HyHEL-10) (HP688307)

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Bioactivity

Detects CD326/EPCAM in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Citatuzumab.
Flow CytoMetry

Flow-cytometry using APC anti-human CD326 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD326 monoclonal antibody (Catalog: HB104016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human CD326 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD326 monoclonal antibody (Catalog: HB104016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD326 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD326 monoclonal antibody (Catalog: HB104016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CD326 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD326 monoclonal antibody (Catalog: HB104016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献

产品说明书

Anti-Human EpCAM/TROP1 Reference Antibody (Citatuzumab, RUO).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

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Anti-Human EpCAM/TROP1 Reference Antibody (Citatuzumab, RUO) (HB104016)

概述

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参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human EpCAM/TROP1 Reference Antibody (Citatuzumab, RUO) (HB104016)

概述

图片

参考文献

产品说明书

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