Anti-Human ErbB3/Her3 Reference Antibody (Duligotuzumab, RUO) (HB116036)

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概述
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概述

货号	HB116036
品牌	abinScience
描述	Duligotuzumab (HB116036) is a research-grade recombinant antibody targeting ERBB3. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
种属反应性	Human
应用	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
同种型	IgG1-kappa
表达系统	Mammalian cells
经测试应用	FCM
靶标	ERBB3, HER3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3, Receptor tyrosine-protein kinase erbB-3
内毒素水平	< 10 EU/mg
纯度	>95% purity as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P21860
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	MEHD7945A, RG-7597, 1314238-96-4
背景	Receptor tyrosine-protein kinase erbB-3 (ERBB3/HER3) is a ~148 kDa protein. Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. May also be activated by CSPG5. Involved in the regulation of myeloid cell differentiation. 1. Ieguchi, K. et al. (2010) The Journal of biological chemistry 285, 31388-98. PMID: 20682778 2. Kinugasa, Y. et al. (2004) Biochemical and biophysical research communications 321, 1045-9. PMID: 15358134 3. Braunstein, EM. et al. (2016) Leukemia 30, 2242-2245. PMID: 27416908
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

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Bioactivity

SEC-HPLC detection for Research Grade Duligotuzumab.
SDS-PAGE

SDS-PAGE for Anti-Human ErbB3/Her3 Reference Antibody (Duligotuzumab, RUO)
Flow CytoMetry

Flow-cytometry using APC anti-human ERBB3 antibody. MCF-7 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human ERBB3 monoclonal antibody (Catalog: HB116036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human ERBB3 antibody. MCF-7 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human ERBB3 monoclonal antibody (Catalog: HB116036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human ERBB3 antibody. HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human ERBB3 monoclonal antibody (Catalog: HB116036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human ERBB3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human ERBB3 monoclonal antibody (Catalog: HB116036) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献

产品说明书

Anti-Human ErbB3/Her3 Reference Antibody (Duligotuzumab, RUO).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

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抗体

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Anti-Human ErbB3/Her3 Reference Antibody (Duligotuzumab, RUO) (HB116036)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ErbB3/Her3 Reference Antibody (Duligotuzumab, RUO) (HB116036)

概述

图片

参考文献

产品说明书

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