Anti-Human CD25/IL-2R alpha Reference Antibody (Daclizumab, RUO) (HF996016)

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概述

货号	HF996016
品牌	abinScience
描述	Daclizumab (HF996016) is a research-grade recombinant antibody targeting CD25. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
种属反应性	Human
应用	ELISA, Bioactivity: FCM, Functional assay, Research in vivo
宿主	Human
克隆类型	Monoclonal
同种型	IgG1-kappa
经测试应用	FCM
靶标	CD25, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, IL2RA, Interleukin-2 receptor subunit alpha, TAC antigen, p55
内毒素水平	< 10 EU/mg
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P01589
状态	Liquid
保存溶液	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	BIIB019, DACHYP, 152923-56-3
背景	Interleukin-2 receptor subunit alpha (CD25/IL2RA) is a ~30 kDa protein. Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

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SEC-HPLC

SEC-HPLC detection for Research Grade Daclizumab.
Bioactivity

Detects Human CD25/IL2RA in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human IL2RA antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human IL2RA monoclonal antibody (Catalog HF996016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Research Grade Daclizumab.
Flow CytoMetry

Flow-cytometry using APC anti-human CD25 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human CD25 monoclonal antibody (Catalog: HF996016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献

产品说明书

Anti-Human CD25/IL-2R alpha Reference Antibody (Daclizumab, RUO).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

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实验相关试剂

Anti-Human CD25/IL-2R alpha Reference Antibody (Daclizumab, RUO) (HF996016)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CD25/IL-2R alpha Reference Antibody (Daclizumab, RUO) (HF996016)

概述

图片

参考文献

产品说明书

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