| 货号 | HS894016 |
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| 品牌 | abinScience |
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| 种属反应性 | Human |
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| 应用 | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
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| 宿主 | Human |
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| 同种型 | IgG1-kappa |
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| 克隆号 | IPH4301 |
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| 生物活性 | IPH4301 induces death of tumor cells expressing MICA/B in vitro via antibody-dependent cellular cytotoxicity and antibody-dependent cellular phagocytosis [1]. IPH4301 blocks the binding of MICA/B to NKG2D, and restores the expression and function of NKG2D on primary human NK cells and T cells [1]. IPH4301 reverses the immunosuppressive effect induced by M2 macrophages and restores the ADCC activity of autologous NK cells against MICA-expressing tumor cell lines [1]. IPH4301-derived PEG-G5.NH2-FITC-DOTA(Gd)-Monalizumab/IPH4301 nanoprobe can efficiently bind to NK-92MI cells. Its binding efficiency and in vitro MRI signal increase with elevated concentrations; besides, it upregulates IFN-γ secretion of NK-92MI cells, enhances the cytotoxicity against MDA-MB-231 triple-negative breast cancer cells and induces tumor cell apoptosis [2]. |
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| 表达系统 | Mammalian cells |
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| 克隆类型 | Monoclonal |
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| 靶标 | MICA, MIC-A, MHC class I polypeptide-related sequence A, PERB11.1, MHC class I polypeptide-related sequence B, MIC-B, MICB, PERB11.2 |
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| 内毒素水平 | < 10 EU/mg |
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| 纯度 | >95% purity as determined by SDS-PAGE. |
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| 纯化方式 | Protein A/G purified from cell culture supernatant. |
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| Accession号 | Q29983, Q29980 |
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| 状态 | Liquid |
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| 保存溶液 |
0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
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| 产品使用信息 |
| 应用方法 |
稀释比例 |
| ELISA |
1:2000-1:20000 |
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| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
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| 别名 | IPH4301 |
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| 背景 | MHC class I polypeptide-related sequence A (MICA/MICB) is a ~42 kDa protein. Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells. Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production. 1. Steinle, A. et al. (2001) Immunogenetics 53, 279-87. PMID: 114915312. Sutherland, CL. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 671-9. PMID: 117779603. Bauer, S. et al. (1999) Science (New York, N.Y.) 285, 727-9. PMID: 10426993 |
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| 文献 | 1. Morel A, et al. Abstract 1491: IPH4301, an antibody targeting MICA and MICB exhibits potent cytotoxic activity and immunomodulatory properties for the treatment of cancer. Cancer Res. 2016 Jul;76(14 Suppl):1491. [HS894016]
2. Wang Z, et al. Preliminary study on PEG-G5.NH2-FITC-DOTA(Gd)-Monalizumab/IPH4301 probe. Biotechnol Bull. 2023;10. [HS894016] |
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| Note | For research use only. Not suitable for clinical or therapeutic use. |
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