| 货号 | HV612076 |
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| 品牌 | abinScience |
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| 种属反应性 | Human |
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| 应用 | ELISA, Bioactivity: FCM, Functional assay, Research in vivo |
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| 宿主 | Human |
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| 克隆类型 | Monoclonal |
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| 同种型 | IgG |
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| 克隆号 | DS-5573a |
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| 生物活性 | DS-5573a (1 ug/mL) binds to the IgC1 and IgC2 domains of human B7-H3 expressed in transfected CHO-K1 cells [1]. The K d value of DS-5573a binding to recombinant human 4IgB7-H3 is 1.8 nM, and the K d value of its binding to recombinant human 2IgB7-H3 is 11 nM [1]. DS-5573a (0.01-1000 ng/mL; 4 h) exerts potent, B7-H3-dependent antibody-dependent cell-mediated cytotoxicity against NCI-H322, MDA-MB-231 and COLO205 cells via human peripheral blood mononuclear cells (PBMC), while exhibits only minimal activity against B7-H3-negative CCRF-CEM cells [1]. DS-5573a (1-10000 ng/mL; 3 h) exerts B7-H3-dependent antibody-dependent cellular phagocytosis (ADCP) activity against NCI-H322, MDA-MB-231 and COLO205 cells via human monocyte-derived macrophages, but shows no activity against B7-H3-negative CCRF-CEM cells [1]. Cell Cytotoxicity Assay [1] Cell Line: NCI-H322, MDA-MB-231, and COLO205 cells Concentration: 0.01, 0.1, 1, 10, 100, 1000 ng/mL Incubation Time: 4 h Result: Exerted potent, B7-H3-dependent ADCC activity against NCI-H322, MDA-MB-231, and COLO205 cells via human PBMCs. DS-5573a (0.003-3 mg/kg; i.p.; once a week; 5 weeks) exhibits dose-dependent, statistically significant antitumor activity against MDA-MB-231 breast adenocarcinoma in female SCID mice, with significant efficacy observed at doses as low as 0.03 mg/kg and maximal activity at 0.3 mg/kg and 3 mg/kg [1]. |
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| 靶标 | 4Ig-B7-H3, B7 homolog 3, B7-H3, B7H3, CD276, CD276 antigen, Costimulatory molecule |
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| 内毒素水平 | < 10 EU/mg |
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| 纯度 | >95% as determined by SDS-PAGE. |
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| 纯化方式 | Protein A/G purified from cell culture supernatant. |
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| Accession号 | Q5ZPR3 |
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| 状态 | Liquid |
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| 保存溶液 |
0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
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| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
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| 别名 | DS-5573a, DS5573a, DS5573a |
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| 背景 | CD276 antigen (B7-H3) is a ~57 kDa protein. May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. CD276 is the therapeutic target of omburtamab (clinical). |
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| 文献 | 1. Nagase-Zembutsu A, et al. Development of DS-5573a: A novel afucosylated mAb directed at B7-H3 with potent antitumor activity. Cancer Sci. 2016;107(5):674-681. [HV612076] |
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| Note | For research use only. Not suitable for clinical or therapeutic use. |
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| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
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