| 货号 | HB863036 |
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| 品牌 | abinScience |
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| 种属反应性 | Human |
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| 应用 | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
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| 宿主 | Human |
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| 同种型 | IgG |
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| 克隆号 | GP369 |
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| 生物活性 | GP369 (0.001-1000 nM, 2-5 days) can significantly inhibit the proliferation of various cells, including FDCP-1, SUM52PE and MCF7 cells [1]. GP369 (0.001-100 nM, 2 days) inhibits FGFR2 IIIb driven proliferation and has no effect on FGFR2 IIIc in FDCP-1 (IC 50 = 1.4 nM) cells [1]. GP369 (30 ug/mL, 3 days) inhibits proliferation induced by FGF7 (25 ng/mL) in MCF7 cells [1]. GP369 (10 ug/mL, 1-48 h) suppresses FGFR2 and ERK1/2 phosphorylation and downstream signaling in Ba/F3 and SNU-16 cells [1]. Cell Proliferation Assay [1] Cell Line: FDCP-1 cells Concentration: 0.001-100 nM Incubation Time: 2 days Result: Significantly inhibited FGFR2 IIIb driven proliferation, including FGFR2 IIIb WT, FGFR2 IIIb N550K and FGFR2 IIIb S252W. Western Blot Analysis [1] Cell Line: SNU-16 cells Concentration: 10 ug/mL Incubation Time: 2, 6, 24 and 48 h Result: Reduced the level of FGFR2 protein. Western Blot Analysis [1] Cell Line: Ba/F3 cells and SNU-16 cells Concentration: 5 ug/mL Incubation Time: Incubated for 1 h and then combinated with FGF7 for 15 min Result: Significantly inhibited FGF7 induced phosphorylation and downstream protein levels, including p-FGFR and p-ERK1/2. GP369 (2-20 mg/kg; i.p.; twice weekly; for 43 days) significantly inhibits tumor growth and reduces FGFR2 phosphorylation and protein levels in C.B-17 severe combined immunodeficient (SCID) mice bearing SNU-16 xenografts [1]. GP369 (20 mg/kg, i.p.; twice weekly; for 27 days) realizes tumor arrest and significantly reduces FGFR2 phosphorylation and protein levels in NCr nude mice bearing MFM-223 xenografts [1]. |
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| 表达系统 | Mammalian cells |
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| 克隆类型 | Monoclonal |
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| 靶标 | K-sam, KSAM, CD332, Keratinocyte growth factor receptor, Fibroblast growth factor receptor 2, FGFR-2, FGFR2, KGFR, BEK |
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| 内毒素水平 | < 10 EU/mg |
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| 纯度 | >95% purity as determined by SDS-PAGE. |
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| 纯化方式 | Protein A/G purified from cell culture supernatant. |
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| Accession号 | P21802 |
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| 状态 | Liquid |
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| 保存溶液 |
0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
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| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
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| 别名 | GP369 |
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| 背景 | Fibroblast growth factor receptor 2 (CD332/FGFR2) is a ~92 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. |
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| 文献 | 1. Bai A, et al. GP369, an FGFR2-IIIb-specific antibody, exhibits potent antitumor activity against human cancers driven by activated FGFR2 signaling. Cancer Res. 2010 Oct 1;70(19):7630-9. [HB863036] |
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| Note | For research use only. Not suitable for clinical or therapeutic use. |
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