| 货号 | HB863056 |
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| 品牌 | abinScience |
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| 种属反应性 | Human |
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| 应用 | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
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| 同种型 | IgG |
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| 克隆号 | HuGAL-FR21 |
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| 生物活性 | HuGAL-FR21 (0.001-1 ug/mL) can specifically bind to FGFR2IIIb (EC 50 approximately 20 pM) and its mutant S252W in SNU-16 cells [1]. HuGAL-FR21 (0.01-100 ug/mL) effectively blocks the binding of FGFR2IIIb (IC 50 approximately 1.5-3 ug/mL) to FGF2, FGF7, and FGF10 [1]. HuGAL-FR21 (10 ug/mL, 30 min) effectively blocks FGF7 induced phosphorylation and significantly inhibits FGF2 induced phosphorylation in SNU-16 cells [1]. HuGAL-FR21 (10 ug/mL, 2-24 h) downregulates FGFR2 protein levels in SNU-16 cells [1]. Western Blot Analysis [1] Cell Line: SNU-16 cells Concentration: 10 ug/mL Incubation Time: 2, 8 and 48 h Result: Reduced the level of FGFR2 protein by approximately 50%. HuGAL-FR21 (0.5-5 mg/kg; i.p.; twice weekly; for 25-30 days) effectively inhibits the growth of tumors and has no obvious toxicity in athymic nude mice bearing SNU-16 xenografts or OCUM-2M xenografts [1]. |
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| 表达系统 | Mammalian cells |
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| 靶标 | K-sam, KSAM, CD332, Keratinocyte growth factor receptor, Fibroblast growth factor receptor 2, FGFR-2, FGFR2, KGFR, BEK |
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| 内毒素水平 | < 10 EU/mg |
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| 纯度 | >95% purity as determined by SDS-PAGE. |
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| 纯化方式 | Protein A/G purified from cell culture supernatant. |
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| Accession号 | P21802 |
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| 状态 | Liquid |
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| 保存溶液 |
0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
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| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
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| 别名 | HuGAL-FR21 |
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| 背景 | Fibroblast growth factor receptor 2 (CD332/FGFR2) is a ~92 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. |
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| 文献 | 1. Zhao WM, et al. Monoclonal antibodies to fibroblast growth factor receptor 2 effectively inhibit growth of gastric tumor xenografts. Clin Cancer Res. 2010 Dec 1;16(23):5750-8. [HB863056] |
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| Note | For research use only. Not suitable for clinical or therapeutic use. |
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| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
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