Human ICAM-1/CD54 Recombinant Protein (C-His) (HY321011)
概述
图片
参考文献
产品说明书
概述
货号
HY321011
品牌
abinScience
描述
Recombinant Human CD54/ICAM1 Protein, C-His (HY321011) expressed in Mammalian Cells, spanning Met1-Glu480. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
P05362
蛋白长度
Met1-Glu480
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
53.08 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH7.4, 1 mM EDTA, 4%trehalose, 1% mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
ICAM1, Major group rhinovirus receptor, CD54, ICAM-1, Intercellular adhesion molecule 1
背景
Intercellular adhesion molecule 1 (CD54/ICAM1) is a ~57 kDa protein. Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL:ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring leukocyte cell-cell adhesion, by providing a calibrated system to namely adjust T-cell killing to the antigen stimulation strength. Also functions as a ligand receptor of the signaling receptor ITGAM:ITGB2/MAC-1 ensuring adhesion between stimulated neutrophils and stimulated endothelial cells. During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. Promotes cell aggregation in epithelial cells through interaction with MUC1.
1. Dustin, ML. et al. (1986) Journal of immunology (Baltimore, Md. : 1950) 137, 245-54. PMID: 3086451
2. Simmons, D. et al. (1988) Nature 331, 624-7. PMID: 3340213
3. Lacouture, C. et al. (2024) Nature communications 15, 407. PMID: 38195629
4. Diamond, MS. et al. (1990) The Journal of cell biology 111, 3129-39. PMID: 1980124
5. van Buul, JD. et al. (2007) The Journal of cell biology 178, 1279-93. PMID: 17875742
6. Hayashi, T. et al. (2001) Digestion 63 Suppl 1, 87-92. PMID: 11173916
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human CD54/ICAM1 Protein, C-His
