Human IL-17RA/IL-17R Recombinant Protein (C-Fc) (HV263011)
概述
图片
参考文献
产品说明书
概述
货号
HV263011
品牌
abinScience
描述
Recombinant Human CD217/IL17RA Protein, C-Fc (HV263011) expressed in Mammalian Cells, spanning Met1-Trp320. Purity: >90% by SDS-PAGE.
Highlights
●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
Q96F46
蛋白长度
Met1-Trp320
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
62.80 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
IL17R, CDw217, IL-17RA, Interleukin-17 receptor A, IL-17 receptor A, IL17RA, CD217
背景
Interleukin-17 receptor A (CD217/IL17RA) is a ~96 kDa protein. Receptor for IL17A and IL17F, major effector cytokines of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. Receptor for IL17A. Receptor for IL17F. Binds to IL17A with higher affinity than to IL17F. Binds IL17A and IL17F homodimers as part of a heterodimeric complex with IL17RC.
1. Kuestner, RE. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 179, 5462-73. PMID: 17911633
2. Yao, Z. et al. (1997) Cytokine 9, 794-800. PMID: 9367539
4. Toy, D. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 177, 36-9. PMID: 16785495
5. Wright, JF. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 181, 2799-805. PMID: 18684971
6. Puel, A. et al. (2011) Science (New York, N.Y.) 332, 65-8. PMID: 21350122
7. Boisson, B. et al. (2013) Immunity 39, 676-86. PMID: 24120361
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human CD217/IL17RA Protein, C-Fc
Bioactivity
Detected by SAA1266 (Catalog No: HV263013) in indirect ELISA.
Bioactivity
Immobilized CS856021 Canine IL17A Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HV263011 Human CD217/IL17RA Recombinant Protein (C-Fc), the EC 50 is 1.71 μg/mL.
