Canine IL-31 Recombinant Protein (C-His) (CV678011)
概述
图片
参考文献
产品说明书
概述
货号
CV678011
品牌
abinScience
描述
Recombinant Dog IL31 Protein, C-His (CV678011) expressed in Mammalian Cells, spanning Ser24-Gln159. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
C7G0W1
蛋白长度
Ser24-Gln159
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Canis lupus familiaris (Dog) (Canis familiaris)
性质
Recombinant
经测试应用
WB
内毒素水平
<0.1 EU/ug as determined by LAL method.
纯度
>90% as determined by SDS-PAGE.
预测分子量
18.27 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
IL-31, Interleukin-31, IL31,
背景
Dovanvetmab (ZTS-00521505) is an IgG1-κ antibody targeting Felcat IL31. Mainly expressed by CHO (Chinese Hamster Ovary) cells. • The IL-4, IL-13 and IL-31 pathways in atopic dermatitis., PMID:34106037 • IL-31-dependent neurogenic inflammation restrains cutaneous type 2 immune response in allergic dermatitis., PMID:37831760 • Interleukin-31: The "itchy" cytokine in inflammation and therapy., PMID:33629401 • A sensory neuron-expressed IL-31 receptor mediates T helper cell-dependent itch: Involvement of TRPV1 and TRPA1., PMID:24373353 • Seladelpar treatment reduces IL-31 and pruritus in patients with primary biliary cholangitis., PMID:38117036 • Nemolizumab: First Approval., PMID:35834124 • Nemolizumab: An Innovative Biologic Treatment to Control Interleukin 31, a Key Mediator in Atopic Dermatitis and Prurigo Nodularis., PMID:35842249 • The Cytokine TGF-β Induces Interleukin-31 Expression from Dermal Dendritic Cells to Activate Sensory Neurons and Stimulate Wound Itching., PMID:32673566 • Pruritus as a Distinctive Feature of Type 2 Inflammation., PMID:33807098 • Targeting Interleukin-31 in Prurigo., PMID:37888922
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Dog IL31 Protein, C-His
Bioactivity
Detects Tirnovetmab in indirect ELISAs.
Bioactivity
Detected by Research Grade Tirnovetmab (CV678016) in indirect ELISAs.
Bioactivity
Detected by Research Grade Anti-Dog IL31 Antibody (SAA1416) (CV678026) in indirect ELISAs.
Western blot
Western blot analysis was performed using anti-dog IL31 monoclonal antibody (Catalog No: CV678013) at 1ug/mL on various samples. Lane 1: recombinant dog IL31
