ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
31.8 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
HLA-Cw, HLA-C, Human leukocyte antigen C, HLAC, HLA class I histocompatibility antigen, C alpha chain
背景
HLA class I histocompatibility antigen, C alpha chain (HLA-C) is a ~40 kDa protein. Antigen-presenting major histocompatibility complex class I (MHCI) molecule with an important role in reproduction and antiviral immunity. In complex with B2M/beta 2 microglobulin displays a restricted repertoire of self and viral peptides and acts as a dominant ligand for inhibitory and activating killer immunoglobulin receptors (KIRs) expressed on NK cells. In an allogeneic setting, such as during pregnancy, mediates interaction of extravillous trophoblasts with KIR on uterine NK cells and regulate trophoblast invasion necessary for placentation and overall fetal growth. During viral infection, may present viral peptides with low affinity for KIRs, impeding KIR-mediated inhibition through peptide antagonism and favoring lysis of infected cells. Presents a restricted repertoire of viral peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-C-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected cells, particularly in chronic viral infection settings such as HIV-1 or CMV infection.
1. Addo, MM. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 1781-6. PMID: 11172028
2. Makadzange, AT. et al. (2010) European journal of immunology 40, 1036-41. PMID: 20104487
3. Fadda, L. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 10160-5. PMID: 20439706
4. Hiby, SE. et al. (2010) The Journal of clinical investigation 120, 4102-10. PMID: 20972337
5. Xiong, S. et al. (2013) The Journal of clinical investigation 123, 4264-72. PMID: 24091323
6. Kaur, G. et al. (2017) Nature communications 8, 15924. PMID: 28649982
7. Hosie, L. et al. (2017) Frontiers in immunology 8, 1776. PMID: 29312307
8. Stewart, CA. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 13224-9. PMID: 16141329
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human HLA-C Protein, N-His
