ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
52.74 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Sialidase-3 (NEU3) is a ~48 kDa protein. Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3. Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR. Desialylates EGFR and activates downstream signaling in proliferating cells. Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes.
1. Wada, T. et al. (1999) Biochemical and biophysical research communications 261, 21-7. PMID: 10405317
2. Monti, E. et al. (2000) The Biochemical journal 349, 343-51. PMID: 10861246
3. Wang, Y. et al. (2001) European journal of biochemistry 268, 2201-8. PMID: 11298736
4. Wang, Y. et al. (2002) The Journal of biological chemistry 277, 26252-9. PMID: 12011038
5. Yamaguchi, K. et al. (2005) The Biochemical journal 390, 85-93. PMID: 15847605
6. Albohy, A. et al. (2010) Glycobiology 20, 1127-38. PMID: 20511247
7. Rodriguez-Walker, M. et al. (2017) Scientific reports 7, 4167. PMID: 28646141
8. Wada, T. et al. (2007) Oncogene 26, 2483-90. PMID: 17334392
9. Mozzi, A. et al. (2015) Glycobiology 25, 855-68. PMID: 25922362
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human NEU3/Sialidase-3 Protein, C-His
