ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
10.42 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Zinc finger protein 36, RNF162A, TIS11A, Growth factor-inducible nuclear protein NUP475, Zfp-36, ZFP36, G0/G1 switch regulatory protein 24, G0S24, Tristetraprolin, NUP475, mRNA decay activator protein ZFP36, TTP
背景
mRNA decay activator protein ZFP36 is a ~34 kDa protein. Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as an 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1, and hence promotes ARE-mediated mRNA deadenylation. Functions also by recruiting components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs. Self regulates by destabilizing its own mRNA.
1. Lai, WS. et al. (1999) Molecular and cellular biology 19, 4311-23. PMID: 10330172
2. Lai, WS. et al. (2000) The Journal of biological chemistry 275, 17827-37. PMID: 10751406
3. Lai, WS. et al. (2001) The Journal of biological chemistry 276, 23144-54. PMID: 11279239
4. Brooks, SA. et al. (2002) Arthritis and rheumatism 46, 1362-70. PMID: 12115244
5. Lai, WS. et al. (2003) Molecular and cellular biology 23, 3798-812. PMID: 12748283
6. Brooks, SA. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 172, 7263-71. PMID: 15187101
7. Ogilvie, RL. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 174, 953-61. PMID: 15634918
8. Marderosian, M. et al. (2006) Oncogene 25, 6277-90. PMID: 16702957
9. Lai, WS. et al. (2006) Molecular and cellular biology 26, 9196-208. PMID: 17030620
10. Kedar, VP. et al. (2010) PloS one 5, e9588. PMID: 20221403
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human ZFP36 Protein, N-His
