Mouse LGMN/Legumain Recombinant Protein (N-His) (MV606011)
概述
图片
参考文献
产品说明书
概述
货号
MV606011
品牌
abinScience
描述
Recombinant Mouse LGMN/Legumain Protein, N-His (MV606011) expressed in Mammalian Cells, spanning Val18-Tyr435. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
O89017
蛋白长度
Val18-Tyr435
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Mus musculus (Mouse)
性质
Recombinant
内毒素水平
Remove endotoxin and sterilization is required for cell assay.
纯度
>90% as determined by SDS-PAGE.
预测分子量
51.42 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Legumain (LGMN) is a ~49 kDa protein. Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol. Required for normal lysosomal protein degradation in renal proximal tubules.
1. Zhao, L. et al. (2014) Cell research 24, 344-58. PMID: 24407422
2. Chen, JM. et al. (1998) The Biochemical journal 335 ( Pt 1), 111-7. PMID: 9742219
3. Chen, JM. et al. (1998) FEBS letters 441, 361-5. PMID: 9891971
4. Rodríguez-Silvestre, P. et al. (2023) Science (New York, N.Y.) 380, 1258-1265. PMID: 37347855
5. Morita, Y. et al. (2007) FEBS letters 581, 1417-24. PMID: 17350006
6. Miller, G. et al. (2011) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25, 1606-17. PMID: 21292981
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Mouse LGMN/Legumain Protein, N-His
