ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Mus musculus (Mouse)
性质
Recombinant
内毒素水平
Remove endotoxin and sterilization is required for cell assay.
纯度
>90% as determined by SDS-PAGE.
预测分子量
31.94 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Legumain is a ~49 kDa protein. Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol. Required for normal lysosomal protein degradation in renal proximal tubules.
1. Zhao, L. et al. (2014) Cell research 24, 344-58. PMID: 24407422
2. Chen, JM. et al. (1998) The Biochemical journal 335 ( Pt 1), 111-7. PMID: 9742219
3. Chen, JM. et al. (1998) FEBS letters 441, 361-5. PMID: 9891971
4. Rodríguez-Silvestre, P. et al. (2023) Science (New York, N.Y.) 380, 1258-1265. PMID: 37347855
5. Morita, Y. et al. (2007) FEBS letters 581, 1417-24. PMID: 17350006
6. Miller, G. et al. (2011) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25, 1606-17. PMID: 21292981
