ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
23.94 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
TMEM70, Transmembrane protein 70, mitochondrial
背景
Transmembrane protein 70, mitochondrial (TMEM70) is a ~28 kDa protein. Scaffold protein that participates in the c-ring assembly of mitochondrial ATP synthase (F(1)F(0) ATP synthase or complex V) by facilitating the membrane insertion and oligomer formation of the subunit c/ATP5MC1 through its interaction. Therefore, participates in the early stage of mitochondrial ATP synthase biogenesis and also protects subunit c/ATP5MC1 against intramitochondrial proteolysis. In addition, binds the mitochondrial proton-transporting ATP synthase complexes I and may play a role in the stability of its membrane-bound subassemblies.
1. Kovalčíkova, J. et al. (2019) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33, 14103-14117. PMID: 31652072
2. Sánchez-Caballero, L. et al. (2020) Biochimica et biophysica acta. Bioenergetics 1861, 148202. PMID: 32275929
3. Bahri, H. et al. (2021) Biochimica et biophysica acta. Molecular cell research 1868, 118942. PMID: 33359711
4. Carroll, J. et al. (2021) Proceedings of the National Academy of Sciences of the United States of America 118. PMID: 33753518
5. Cízková, A. et al. (2008) Nature genetics 40, 1288-90. PMID: 18953340
6. Hejzlarová, K. et al. (2011) Biochimica et biophysica acta 1807, 144-9. PMID: 20937241
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human TMEM70 Protein, N-His-SUMO
