ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
14.77 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
SUZ12, Polycomb protein SUZ12, Joined to JAZF1 protein, CHET9, KIAA0160, ChET 9 protein, Suppressor of zeste 12 protein homolog, Chromatin precipitated E2F target 9 protein, JJAZ1
背景
Polycomb protein SUZ12 is a ~83 kDa protein. Polycomb group (PcG) protein. Component of the PRC2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
1. Cao, R. et al. (2004) Molecular cell 15, 57-67. PMID: 15225548
2. Kirmizis, A. et al. (2004) Genes & development 18, 1592-605. PMID: 15231737
3. Pasini, D. et al. (2004) The EMBO journal 23, 4061-71. PMID: 15385962
4. Bracken, AP. et al. (2006) Genes & development 20, 1123-36. PMID: 16618801
5. Bracken, AP. et al. (2007) Genes & development 21, 525-30. PMID: 17344414
6. Sarma, K. et al. (2008) Molecular and cellular biology 28, 2718-31. PMID: 18285464
7. Imagawa, E. et al. (2017) Human mutation 38, 637-648. PMID: 28229514
8. Chen, S. et al. (2018) Molecular cell 69, 840-852.e5. PMID: 29499137
9. Chen, S. et al. (2020) Molecular cell 77, 1265-1278.e7. PMID: 31959557
10. Cao, R. et al. (2002) Science (New York, N.Y.) 298, 1039-43. PMID: 12351676
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human SUZ12 Protein, N-His
