ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
42.82 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Calcium-transporting ATPase type 2C member 1, ATP-dependent Ca(2+) pump PMR1, PMR1L, KIAA1347, ATP2C1, ATPase 2C1
背景
Calcium-transporting ATPase type 2C member 1 (ATP2C1) is a ~100 kDa protein. ATP-driven pump that supplies the Golgi apparatus with Ca(2+) and Mn(2+) ions, both essential cofactors for processing and trafficking of newly synthesized proteins in the secretory pathway. Within a catalytic cycle, acquires Ca(2+) or Mn(2+) ions on the cytoplasmic side of the membrane and delivers them to the lumenal side. The transfer of ions across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state. Plays a primary role in the maintenance of Ca(2+) homeostasis in the trans-Golgi compartment with a functional impact on Golgi and post-Golgi protein sorting as well as a structural impact on cisternae morphology. Responsible for loading the Golgi stores with Ca(2+) ions in keratinocytes, contributing to keratinocyte differentiation and epidermis integrity.
1. Fairclough, RJ. et al. (2003) The Journal of biological chemistry 278, 24721-30. PMID: 12707275
2. Dode, L. et al. (2005) The Journal of biological chemistry 280, 39124-34. PMID: 16192278
3. Lissandron, V. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 9198-203. PMID: 20439740
4. Mukhopadhyay, S. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 858-63. PMID: 21187401
5. Chen, J. et al. (2019) The Journal of biological chemistry 294, 7878-7891. PMID: 30923126
6. Dode, L. et al. (2006) The Journal of biological chemistry 281, 3182-9. PMID: 16332677
7. Behne, MJ. et al. (2003) The Journal of investigative dermatology 121, 688-94. PMID: 14632183
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human ATP2C1 Protein, C-His
