HIV-1 gp120 Recombinant Protein (C-His) (VK617011)
概述
图片
参考文献
产品说明书
概述
货号
VK617011
品牌
abinScience
描述
Recombinant HIV1 GP140 Protein, C-His (VK617011) expressed in Mammalian Cells, spanning Met23-Ile680. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
Q78225
蛋白长度
Met23-Ile680
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Human immunodeficiency virus type 1 (HIV-1)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
85.10 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Envelope glycoprotein gp160, Env polyprotein, Surface protein gp120, SU, Glycoprotein 120, gp120, Transmembrane protein gp41, TM, Glycoprotein 41, gp41, env
背景
Envelope glycoprotein gp160 (gp140/Glycoprotein 140) is a ~97 kDa protein. Envelope glycoprotein gp160: Oligomerizes in the host endoplasmic reticulum into predominantly trimers. In a second time, gp160 transits in the host Golgi, where glycosylation is completed. The precursor is then proteolytically cleaved in the trans-Golgi and thereby activated by cellular furin or furin-like proteases to produce gp120 and gp41.
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant HIV1 GP140 Protein, C-His
Bioactivity
Detects Zinlirvimab in indirect ELISAs.
Bioactivity
Detects Elipovimab in indirect ELISAs.
Bioactivity
Detects Iv0111 in indirect ELISAs.
Bioactivity
Detected by Research Grade Anti-HIV1 gp120/Glycoprotein 120 (VRC01LS) (Catalog No: VK617066) in indirect ELISA.
