ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Streptococcus pyogenes
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
134.61 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Endo-beta-N-acetylglucosaminidase
背景
Endo-beta-N-acetylglucosaminidase EndoS (Endo S) is a ~111 kDa protein. Endoglucosidase that acts as a host immune evasion factor by mediating hydrolysis of the N-linked glycan from the Fc region of host immunoglobulin-gamma (IgG) during infection. Specifically catalyzes the hydrolysis of the beta-1,4 linkage between the first two N-acetylglucosamine residues of the complex-type N-linked glycan located on 'Asn-297' of the Fc region of IgG antibodies (IGHG1, IGHG2, IGHG3 or IGHG4), thereby preventing interaction between IgGs and Fc receptors and ability to activate the complement pathway. Shows a specificity for biantennary complex type N-glycans; does neither cleave larger complex type glycans nor oligomannose and nor hybrid-type glycans. Specifically acts on IgGs; does not act on immunoglobulin alpha, beta, delta or mu.
1. Collin, M. et al. (2001) The EMBO journal 20, 3046-55. PMID: 11406581
2. Collin, M. et al. (2001) Infection and immunity 69, 7187-9. PMID: 11598100
3. Collin, M. et al. (2002) Infection and immunity 70, 6646-51. PMID: 12438337
4. Allhorn, M. et al. (2008) BMC microbiology 8, 3. PMID: 18182097
5. Allhorn, M. et al. (2010) Blood 115, 5080-8. PMID: 20357243
6. Sjögren, J. et al. (2011) BMC microbiology 11, 120. PMID: 21619648
7. Goodfellow, JJ. et al. (2012) Journal of the American Chemical Society 134, 8030-3. PMID: 22551167
8. Huang, W. et al. (2012) Journal of the American Chemical Society 134, 12308-18. PMID: 22747414
9. Dixon, EV. et al. (2014) The Journal of biological chemistry 289, 13876-89. PMID: 24668806
10. Trastoy, B. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 6714-9. PMID: 24753590
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Streptococcus pyogenes EndoS Protein, N-GST
