Recombinant Human CA4 Protein, N-His (PX-P5526) expressed in E. coli, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
E. coli Expression — Optimized for functional studies.
表达系统
E. coli
Accession号
P22748
蛋白长度
Ala19-Lys283
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
32.55 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Carbonic anhydrase 4, Carbonate dehydratase IV, CA-IV, CA4, Carbonic anhydrase IV
背景
Carbonic anhydrase 4 (CA4) is a ~35 kDa protein. Catalyzes the reversible hydration of carbon dioxide into bicarbonate and protons and thus is essential to maintaining intracellular and extracellular pH. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.
1. Yang, Z. et al. (2005) Human molecular genetics 14, 255-65. PMID: 15563508
2. Temperini, C. et al. (2006) Journal of medicinal chemistry 49, 3019-27. PMID: 16686544
3. Temperini, C. et al. (2006) Chemistry (Weinheim an der Bergstrasse, Germany) 12, 7057-66. PMID: 16807956
4. Temperini, C. et al. (2007) Bioorganic & medicinal chemistry letters 17, 628-35. PMID: 17127057
5. Temperini, C. et al. (2007) Bioorganic & medicinal chemistry letters 17, 2210-5. PMID: 17314045
