Recombinant Mouse HMGB1 Protein, N-His (PX-P5985) expressed in Mammalian Cells, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
Mammalian Cells Expression — Optimized for functional studies.
表达系统
Mammalian Cells
Accession号
P63158
蛋白长度
Met1-Glu215
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Mus musculus (Mouse)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
27 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
High mobility group protein B1, HMGB1, HMG1, HMG-1, High mobility group protein 1
背景
High mobility group protein B1 (HMGB1) is a ~24 kDa protein. Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as a sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy.
1. Yang, H. et al. (2013) Journal of leukocyte biology 93, 865-73. PMID: 23446148
2. Li, G. et al. (2013) Frontiers in immunology 4, 68. PMID: 23519706
3. Li, G. et al. (2013) Seminars in cancer biology 23, 380-90. PMID: 23994764
4. Lee, SA. et al. (2014) Yonsei medical journal 55, 1165-76. PMID: 25048472
5. Mitola, S. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 12-5. PMID: 16365390
