ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
24.50 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Nuclear protein 1; Candidate of metastasis 1; Protein p8; NUPR1; COM1
背景
Nuclear protein 1 is a ~8 kDa protein. Transcription regulator that converts stress signals into a program of gene expression that empowers cells with resistance to the stress induced by a change in their microenvironment. Thereby participates in the regulation of many processes namely cell-cycle, apoptosis, autophagy and DNA repair responses. Controls cell cycle progression and protects cells from genotoxic stress induced by doxorubicin through the complex formation with TP53 and EP300 that binds CDKN1A promoter leading to transcriptional induction of CDKN1A. Protects pancreatic cancer cells from stress-induced cell death by binding the RELB promoter and activating its transcription, leading to IER3 transactivation. Negatively regulates apoptosis through interaction with PTMA.
1. Encinar, JA. et al. (2001) The Journal of biological chemistry 276, 2742-51. PMID: 11056169
2. Hoffmeister, A. et al. (2002) The Journal of biological chemistry 277, 22314-9. PMID: 11940591
3. Malicet, C. et al. (2006) Biochemical and biophysical research communications 339, 284-9. PMID: 16300740
4. Malicet, C. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 2671-6. PMID: 16478804
5. Clark, DW. et al. (2008) Current cancer drug targets 8, 421-30. PMID: 18690848
6. Gironella, M. et al. (2009) Journal of cellular physiology 221, 594-602. PMID: 19650074
7. Sambasivan, R. et al. (2009) Journal of cell science 122, 3481-91. PMID: 19723804
8. Kong, DK. et al. (2010) Molecular biology of the cell 21, 1335-49. PMID: 20181828
9. Hamidi, T. et al. (2012) The Journal of clinical investigation 122, 2092-103. PMID: 22565310
10. Vincent, AJ. et al. (2012) FEBS letters 586, 3429-34. PMID: 22858377
Note
For research use only
图片
SDS-PAGE
SDS-PAGE for Recombinant Human NUPR1 Protein, N-His-KSI
