MERS-CoV ORF4a Recombinant Protein (N-His) (VK073052)

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概述

货号	VK073052
品牌	abinScience
描述	Recombinant MERS-CoV ORF4a Protein, N-His (VK073052) expressed in E. coli, spanning Met1-Asn109. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
表达系统	E. coli
Accession号	K9N4V0
蛋白长度	Met1-Asn109
应用	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属	Middle East respiratory syndrome-related coronavirus (MERS-CoV)
性质	Recombinant
内毒素水平	Please contact with the lab for this information.
纯度	>90% as determined by SDS-PAGE.
预测分子量	14.40 kDa
状态	Lyophilized
保存溶液	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	Non-structural protein ORF4a; ORF4a; ORF4a
背景	Non-structural protein ORF4a is a ~12 kDa protein. DsRNA-binding protein that plays a role in the inhibition of host innate response. Suppresses host PACT-induced activation of RIGI and MDA5 and thereby circumvents the production of type I interferons. Also prevents the activation of host NF-kappa-B. Inhibits the integrated stress response (ISR) in the infected cell by binding to dsRNA and inhibiting EIF2AK2-mediated phosphorylation of EIF2S1/eIF2-alpha. Stress granule formation is thus inhibited, which allows protein synthesis and viral replication. 1. Niemeyer, D. et al. (2013) Journal of virology 87, 12489-95. PMID: 24027320 2. Yang, Y. et al. (2013) Protein & cell 4, 951-61. PMID: 24318862 3. Siu, KL. et al. (2014) Journal of virology 88, 4866-76. PMID: 24522921 4. Nakagawa, K. et al. (2018) Journal of virology 92. PMID: 30068649
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant MERS-CoV ORF4a Protein, N-His

参考文献

产品说明书

MERS-CoV ORF4a Recombinant Protein (N-His).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

MERS-CoV ORF4a Recombinant Protein (N-His) (VK073052)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

MERS-CoV ORF4a Recombinant Protein (N-His) (VK073052)

概述

图片

参考文献

产品说明书

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